qPCR assays with dual-labeled probes for genotyping honey bee variants associated with varroa resistance

Abstract Background The varroa mite is one of the main causes honey bee mortality. An important mechanism by which bees increase their resistance against this expression suppressed reproduction. This trait describes physiological inability mites to produce viable offspring and was found associated with eight genomic variants in previous research. Results paper presents development validation high-throughput qPCR assays dual-labeled probes for discriminating these single-nucleotide variants. Amplicon sequences used assay revealed additional primer/probe binding sites four out assays. As two interfered genotyping outcome supplementary primers and/or were developed. Inclusion mixes allowed correct all interest within our population. Conclusion These outcomes underline importance checking interfering designing Ultimately, availability allows reproduction paves way marker assisted selection breeding programs.